Simulated fluorescence process algorithm: Difference between revisions
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{{Short description|Computing algorithm}} |
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The '''Simulated Fluorescence Process''' (SFP) is a computing [[algorithm]] used for [[scientific visualization]] of [[3D scanning|3D data]] from, for example, [[fluorescence microscope]]s. By [[Computer simulation|modeling]] a physical light/matter interaction process, an image can be computed which shows the data as it would have appeared in reality when viewed under these conditions. |
The '''Simulated Fluorescence Process''' (SFP) is a computing [[algorithm]] used for [[scientific visualization]] of [[3D scanning|3D data]] from, for example, [[fluorescence microscope]]s. By [[Computer simulation|modeling]] a physical light/matter interaction process, an image can be computed which shows the data as it would have appeared in reality when viewed under these conditions. |
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==Principle== |
==Principle== |
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The algorithm considers a virtual light source producing excitation light that illuminates the object. This casts shadows either on parts of the object itself or on other objects below it. The interaction between the excitation light and the object provokes the emission light, which also interacts with the object before it finally reaches the eye of the viewer. |
The algorithm considers a virtual light source producing excitation light that illuminates the object. This casts shadows either on parts of the object itself or on other objects below it. The interaction between the excitation light and the object provokes the emission light, which also interacts with the object before it finally reaches the eye of the viewer.<ref>{{Cite journal |last=Voort |first=H. T. M. |last2=Brakenhoff |first2=G. J. |last3=Baarslag |first3=M. W. |date=1989-02 |title=Three-dimensional visualization methods for confocal microscopy |url=http://dx.doi.org/10.1111/j.1365-2818.1989.tb00553.x |journal=Journal of Microscopy |volume=153 |issue=2 |pages=123–132 |doi=10.1111/j.1365-2818.1989.tb00553.x |issn=0022-2720}}</ref><ref>{{Cite journal |last=Noordmans |first=H.J. |last2=van der Voort |first2=H.T.M. |last3=Smeulders |first3=A.W.M. |date=2000 |title=Spectral volume rendering |url=http://dx.doi.org/10.1109/2945.879782 |journal=IEEE Transactions on Visualization and Computer Graphics |volume=6 |issue=3 |pages=196–207 |doi=10.1109/2945.879782 |issn=1077-2626}}</ref> |
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== See also == |
== See also == |
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==References== |
==References== |
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{{Reflist}} |
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H. T. M. van der Voort, G. J. Brakenhoff and M. W. Baarslag. "[https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2818.1989.tb00553.x Three-dimensional visualization methods for confocal microscopy]", Journal of Microscopy, Vol. 153, Pt 2, February 1989, pp. 123–132. |
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Noordmans, Herke Jan, Hans TM van der Voort, and Arnold WM Smeulders. "[https://ieeexplore.ieee.org/abstract/document/879782/ Spectral volume rendering]." IEEE transactions on visualization and computer graphics 6.3 (2000): 196–207. |
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==External links== |
==External links== |
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[[Category:Microscopy]] |
[[Category:Microscopy]] |
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[[Category:Fluorescence]] |
[[Category:Fluorescence]] |
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Revision as of 20:02, 3 March 2024
 | This article includes a list of references, related reading, or external links, but its sources remain unclear because it lacks inline citations. (December 2010) |
The Simulated Fluorescence Process (SFP) is a computing algorithm used for scientific visualization of 3D data from, for example, fluorescence microscopes. By modeling a physical light/matter interaction process, an image can be computed which shows the data as it would have appeared in reality when viewed under these conditions.
Principle
The algorithm considers a virtual light source producing excitation light that illuminates the object. This casts shadows either on parts of the object itself or on other objects below it. The interaction between the excitation light and the object provokes the emission light, which also interacts with the object before it finally reaches the eye of the viewer.[1][2]
See also
References
- ^ Voort, H. T. M.; Brakenhoff, G. J.; Baarslag, M. W. (1989-02). "Three-dimensional visualization methods for confocal microscopy". Journal of Microscopy. 153 (2): 123–132. doi:10.1111/j.1365-2818.1989.tb00553.x. ISSN 0022-2720.
{{cite journal}}: Check date values in:|date=(help) - ^ Noordmans, H.J.; van der Voort, H.T.M.; Smeulders, A.W.M. (2000). "Spectral volume rendering". IEEE Transactions on Visualization and Computer Graphics. 6 (3): 196–207. doi:10.1109/2945.879782. ISSN 1077-2626.
External links
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