U.S. Highway 129 is a spur of U.S. Highway 29. It currently runs for 582 miles from Knoxville, Tennessee to Chiefland, Florida at U.S. Highway 19 and U.S. Highway 98. It passes through the states of Tennessee, North Carolina, Georgia and Florida. It goes through the cities of Knoxville, Tennessee, Athens, Georgia, and Macon, Georgia.