Aminoglycoside N6'-acetyltransferase

In enzymology, an aminoglycoside N6'-acetyltransferase (EC 2.3.1.82) is an enzyme that catalyzes the chemical reaction

acetyl-CoA + kanamycin-B $\rightleftharpoons$ CoA + N₆'-acetylkanamycin-B

Thus, the two substrates of this enzyme are acetyl-CoA and kanamycin B, whereas its two products are CoA and N6'-acetylkanamycin-B.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:kanamycin-B N6'-acetyltransferase. Other names in common use include aminoglycoside 6'-N-acetyltransferase, aminoglycoside-6'-acetyltransferase, aminoglycoside-6-N-acetyltransferase, and kanamycin acetyltransferase.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1S3Z, 1S5K, 1S60, and 2A4N.

References

• Le Goffic F, Martel A (1974). "[Resistance to aminosides induced by an isoenzyme, kanamycin acetyltransferase]". Biochimie. 56: 893–7. PMID 4614862.
• Benveniste R, Davies J (1971). "Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor". Biochemistry. 10: 1787–96. doi:10.1021/bi00786a009. PMID 4935296.
• Dowding JE (1977). "Mechanisms of gentamicin resistance in Staphylococcus aureus". Antimicrob. Agents. Chemother. 11: 47–50. PMID 836013.

External links

The CAS registry number for this enzyme class is Template:CAS registry.