Microphysiometry
 | This article, Microphysiometry, has recently been created via the Articles for creation process. Please check to see if the reviewer has accidentally left this template after accepting the draft and take appropriate action as necessary.
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| This article, Microphysiometry, has recently been created via the Articles for creation process. Please check to see if the reviewer has accidentally left this template after accepting the draft and take appropriate action as necessary.
Reviewer tools: Inform author |
| This article, Microphysiometry, has recently been created via the Articles for creation process. Please check to see if the reviewer has accidentally left this template after accepting the draft and take appropriate action as necessary.
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Comment: Needs a lead sentence to explain what it is, also when was it coined, who coined it, and how widespread the term has become. AngusWOOF (bark • sniff) 19:43, 30 April 2019 (UTC)
- Comment: Now it reads more like a dictionary definition AngusWOOF (bark • sniff) 00:41, 30 April 2019 (UTC)
- Comment: I would like to see a few more independent and reliable sources (instead of just one). Perhaps there may be more articles in journals which may be useful. It may be also a good idea to expand the information you have written in the draft with the sources you find. Thanks for your work anyway! Dreamy Jazz 🎷 talk to me | my contributions 19:48, 26 April 2019 (UTC)
Microphysiometry is the In-vitro measurement of the functions and activities of life or of living matter (as organs, tissues, or cells) and of the physical and chemical phenomena involved on a very small (micrometer) scale.[1] [2] The term microphysiometry emerged in the scientific literature at the end of the 1980s.[3] [4]
The primary parameters assessed in microphysiometry comprise pH and the concentration of dissolved oxygen, glucose, and lactate, with an emphasis on the first two. Measuring these parameters experimentally in combination with a fluidic system for cell culture maintenance and a defined application of drugs or toxins provides the quantitative output parameters extracellular acidification rates (EAR), oxygen consumption rates (OUR), and rates of glucose consumption or lactate release to characterize the metabolic situation. Due to the label-free nature of sensor-based measurements, dynamic monitoring of cells or tissues for several days or even longer is feasible. On an extended timescale, a dynamic analysis of a cell’s metabolic response to an experimental treatment can distinguish acute effects (e.g., 1 h after a treatment), early effects (e.g., at 24 h), and delayed, chronic responses (e.g., at 96 h). As stated by Alajoki et al. “The concept is that it is possible to detect receptor activation and other physiological changes in living cells by monitoring the activity of energy metabolism”[5]
References
- ^ McConnel HM, Owicki JC, Parce JW, Miller DL, Baxter GT, Wada HG, Pitchford S (1992). "The Cytosensor Microphysiometer: Biological Applications of Silicon Technology", Science, 257, 1906-1912
- ^ Brischwein, M.; Wiest, J. (2018). "Microphysiometry". Bioanalytical Reviews. Springer. doi:10.1007/11663_2018_2.
- ^ Hafeman DG, Parce JW, McConnell H (1988). "Light-addressable potentiometric sensor for biochemical systems", Science 240, 1182–1185
- ^ Owicki JC, Parce JW (1990). "Bioassays with a microphysiometer". Nature 344, 271–272
- ^ Alajoki ML, Bayter GT, Bemiss WR, Blau D, Bousse LJ, Chan SDH, Dawes TD, Hahnenberger KM, Hamilton JM, Lam P, McReynolds RJ, Modlin DN, Owicki C, Parce JW, Redington D, Stevenson K, Wada HG, Williams J (1997). "High-performance microphysiometry in drug discovery", Devlin JP (ed) High throughput screening – the discovery of bioactive substances. Marcel Dekker, New York, 427–442.
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