EcoRI
In molecular biology, EcoRI (pronounced "Eco R One") is a commonly used restriction enzyme. It creates sticky ends with 5' end overhangs. The nucleic acid sequence where the enzyme cuts is G|AATTC, which is a palindrome as the complementary sequence is CTTAA|G.
EcoRI, which is isolated from certain strains of E. coli, is often used to cut open a plasmid vector to insert a gene-of-interest during gene cloning. EcoRI is prone to exhibit star activity if an excess of salts are present during the digestion.
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